Focus of the workshop will include species of interest to APSA members, in-house development of vigour tests and the application of new technologies. This programme covers four of the validated vigour tests, Electrical Conductivity (EC), Accelerated Ageing (AA), Controlled deterioration (CD) and Radicle Emergence (RE). The workshop will describe the current validated test methods and how the conditions for the test were established. Practical work will apply the validated test methods to species of interest to APSA members, using test conditions selected from research papers in which the validated tests have been applied to sweetcorn and species from the Solanaceae and Cucurbitaceae. The test results will be used to describe how appropriate test conditions are determined. Important aspects of test development will be discussed, including field and glasshouse trials. In addition, we shall show how image analysis can be applied in vigour testing. In addition to lectures and practicals, participants will provide information based on their experience which will form the basis of discussions.
| TIME (GMT+7) | AGENDA | SPEAKER |
| 08:00 – 08:30 |
Registration & Opening workshop session |
APSA / Lecturers |
| 08:30 – 10:00 |
Introduction to vigour: examples, ageing as a cause of reduced vigour. Q&A Session |
Dr Alison A. Powell |
| 10:00 – 10:30 |
Morning Break |
|
| 10:30 – 12:00 |
CD, illustration of the test method and test results including how test conditions are selected |
Marie-Helene Wagner |
| 12:00 – 13:00 |
Lunch |
|
| 13:00 – 15:00 |
Practical on Controlled deterioration (CD) test: participants evaluate test results for melon, tomato and sweet pepper Calculation, Report and Discussion |
Lecturers / staffs |
| 15:00 – 15:30 |
Afternoon Break |
|
| 15:30 – 17:00 |
Discussion on application of vigour tests within participants work experience Workshop Day 1 summary |
Lecturers/participants Dr Alison A. Powell |
| TIME (GMT+7) | AGENDA | SPEAKER |
| 08:30 – 10:00 |
Electrical conductivity (EC) test: illustration of test method and test results: including how test conditions selected |
Dr Hulya Ilbi |
| 10:00 – 10:30 |
Morning Break |
|
| 10:30 – 12:00 |
Practical on EC test: participants set up the EC test for melon using several different test conditions |
Lecturers/staff |
| 12:00 – 13:00 |
Lunch |
|
| 13:00 – 14:00 |
Radicle emergence : illustration of test method and test results: including how test conditions selected |
Dr Stan Matthews |
| 14:00 – 15:00 |
Automation of radicle emergence assessment (RGB imaging and multispectral imaging) |
Marie-Helene Wagner |
| 15:00 – 15:30 |
Afternoon Break |
|
| 15:30 – 17:00 |
Practical on RE test: participants evaluate RE test for radish, sweetcorn and cucumber. Calculation, Report, Discussion of Radicle Emergence (RE) test |
Lecturers / Staff |
| TIME (GMT+7) | AGENDA | SPEAKER |
| 08:30 – 10:00 |
Practical on EC test in radish and melon : Take EC measurements from tests set up on day 2. |
Lecturers / Staff |
| 10:00 – 10:30 |
Morning Break |
|
| 10:30 – 12:00 |
AA: illustration of test method and test results: including how test conditions selected. Potential use of Saturated Salt Accelerated Ageing test |
Dr Alison A. Powell |
| 12:00 – 13:00 |
Lunch |
|
| 13:00 – 14:00 |
Practical on AA test in cucumber: participants assess test results. |
Lecturers / Staff |
| 14:00 – 15:00 |
Ageing/ repair hypothesis Discussion on selection of an appropriate test |
Dr Alison A. Powell All participants |
| 15:00 – 15:30 |
Afternoon Break |
|
| 15:30 – 17:00 |
How to develop a test for a new species
|
Dr Alison A. Powell |
Dr Alison Powell (Chair, ISTA Vigour Committee, Aberdeen University, UK)Background
Dr Hulya Ilbi (Vice Chair ISTA Vigour Committee, Ege University, Turkey)
Dr Stan Matthews (Vigour Committee member, Aberdeen University, UK)Background
Current position
Teaching and Research experience
Research interests
Marie-Helene Wagner (Vigour Committee member, GEVES, France)Biography:
Marie-Hélène Wagner obtained a MSC in Plant Biology from University of Paris (Paris VII). She is currently a seed physiologist at the National Seed Testing Station from GEVES in Angers, France. She has 29 years of experience in developing seed testing methods related to seed physiology and based in seed science for agricultural, forage and vegetable seeds. She has been a member of the ISTA Vigour Testing Committee since 2004 and contributed to three ISTA workshop on vigour testing (Angers, 2006, Melbourne, 2012, Bangkok, 2020). She has developed several methods on oilseed rape including biochemical markers research through 15 years of collaborative projects. She has contributed to the validation of automated tools for scoring germination using computer vision which can now be used for more than forty species. She has participated to the international standardisation and validation of the controlled deterioration test on Brassica and thereafter shortened the method to increase its use by seed companies. For two years, she has been involved in interactions between seed and microorganisms and several projects with breeders to phenotype germination of large collections of cultivars of sugar beet or sunflower have been completed.
Abstract:
From seed longevity to vigour tests: assessments of seed vigour based on germination time progress curves and artificial ageing.
For many crops, seed ageing is a main factor in seed quality loss and plays a key role in determining vigour. Seed of any species is at its highest level of vigour and germination potential at physiological maturity. Then, a continuous and irreversible process of deterioration begins and progresses until the seed loses its capacity to germinate. Accelerated ageing test and controlled deterioration test were developed first to predict seed longevity and used for modelling viability curves but they could correlate also to field emergence especially in stressful conditions. Using image analysis, delay of germination due to seed ageing can be quantified and allows developing radicle emergence tests for several species.